Fig 1: Circ_0000479 was upregulated in MH7A cells. A Relative expression of circ_0000479 in MH7A cells and normal FLSs. B Relative expression of EPSTI1 in MH7A cells and normal FLSs. C The expression of EPSTI1 protein in MH7A cells and normal FLSs. D Relative RNA levels of circ_0000479 and EPSTI1 after RNase R treatment. E Analysis for RNA abundance of circ_0000479 and EPSTI1 after Actinomycin D treatment at indicated time points. F Detected by qRT-PCR, circ_0000479 was mainly enriched in the cytoplasm. **P < 0.01; ****P < 0.0001
Fig 2: Effects of circ_0000479 on MH7A cell progression. A The screening of interfering fragments of circ_0000479 by qRT-PCR. B Relative expression of EPSTI1 in MH7A cells transfected with si-circ_0000479 or si-NC. C The expression of EPSTI1 protein in MH7A cells transfected with si-circ_0000479 or si-NC. D CCK-8 assays detected the OD value of MH7A cells transfected with si-circ_0000479 or si-NC. E The apoptotic ratio of MH7A cells transfected with si-circ_0000479 or si-NC by flow cytometry analysis. F Transwell assays detected the number of invaded MH7A cells after transfection of si-circ_0000479 or si-NC. Magnification: 100 × . G The wound healing ratio of MH7A cells after transfection of si-circ_0000479 or si-NC. Magnification: 40 × . H The positive expression of TNF-a, IL-6 and IL-1ß in MH7A cells transfected with si-circ_0000479 or si-NC by ELISA kits. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001
Supplier Page from Abcam for Anti-EPSTI1 antibody